Production Of Transgenic Animals Notes

A transgenic animal is an animal in which one or more genes have been introduced into its nonreproductive cells.

Production of transgenic animals notes. Microinjection blastocyst injection and using a retrovirus Nuclear transfers Artificial chromosomes for gene transfer PRODUCTION OF TRANSGENIC ANIMALS THE METHODOLOGY. Transgenic mice are developed by injecting DNA into the oocytes or 1-2 celled embryos taken from female mice. Genetically modified animals are proving ever more vital in.

In addition to the gene itself the DNA usually includes other sequences to enable it to be incorporated into the DNA of the host and to be expressed correctly by the cells of the host. Due to the controversial concept of cloning and scientific testing on animals there are many questions that revolve around this topic. Transgenic animals are being used to study gene mechanism and function to adapt pig organs to be transferred to humans to prepare recombinant pharmaceutical proteins in milk and egg white and to improve breeding and food production.

The first transgenic animal was produced in 1983 when genes for human growth hormone were introduced into mice. The transgenic expression is not only used to breed animals which have specific traits but also to breed animals that are lacking specific genes This technique is called as knockout or gene. Then they are inserted into a blastocyst and implanted into a hosts uterus to grow normally.

Milk as the Medium of Protein Production. Transgenic organisms are organisms that are injected with foreign DNA. PowerPoint PPT presentation.

Theses mechanism is absent in cell culture. After injecting the DNA the embryo is implanted into the uterus of receptive females. One favoured method involves the inoculation of the DNA into the pronucleus of a fertilised ovum followed by implantation into pseudo pregnant females.

Advantages of Transgenic Animals. Transgene is incorporated into the ES cell by Microinjection By a retro virus By electroporation Transgenic stem cells are grown in vitro. 43 Creation of the transgenic animal A number of methods are currently in use for the creation of transgenic animals.